pcDNA3.1(+) 载体

2022-03-31 11:04:00
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基本信息

质粒类型: 哺乳动物表达载体
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5428 bp
5' 测序引物:   pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物:   pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin / G418

质粒图谱

pcDNA3.1(+) 质粒图谱

载体描述

pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  • Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
  • Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
  • Neomycin resistance gene for selection of stable cell lines
  • Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.

This pcDNA™3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family

Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in either forward (+) or reverse (-) orientations
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)

• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

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